Current Courses:
Mass Spectrometry of Biomolecules
We are interested in using our state of the art linear ion trap equipped with a nano HPLC for the determination of protein sequence of various biologically relevant proteins from the organism ciox larcyma-jobi (Job's tears). There are extensive protein and DNA databases available for use in proteomics and genomics. Using these databases, theoretical fragments of protein cleavage can be created and then compared to the experimental masses of known peptide fragments. This technique allows proteins to be assigned "scores" depending on the sequence coverage and number of "hits"
In addition to protein characterization we are interested in further investigating the non-covalent interactions of biologically relevant models. Ion trap mass spectrometry allows the investigation of the thermochemistry of the non-covalent interactions including the strength of the non-covalent bond between crown ethers and amines. Understanding the structure, dynamics, and strength of these interactions can give great insight to the nature of binding, structural configuration, and function of a non-covalent complex. If thermochemical information, such as binding energy and reactivity, is known about a system, it may facilitate investigations of structure and biological function, which are not well understood. In addition to experimental approaches, semi-empirical and ab initio calculations of geometry and binding energies can be utilized in the supplementation and validation of the results
Publications and Presentations:
- Jellen, E.E., Ryzhov, V. Probing the Stability and Structure of Metalloporphyrin Complexes with Basic Peptides by Mass Spectrometry. European Journal of Mass Spectrometry: 10.1255/ejms. 704
- Jellen, E.E.; Chappell, A. M.; Ryzhov, V. Effects of Size of Non-Covalent Complexes on the Stability During Collision- Induced Dissociation. Rapid Communications of Mass Spectrometry, 2002; 16 (19): 1799-1804.
- Hayes, L. A.; Chappell, A. M.; Jellen, E. E.; Ryzhov, V. Binding of Metalloporphyrins to Model Nitrogen Bases: Collision-Induced Dissociation and Ion-Molecule Reactions Studies. International Journal of Mass Spectrometry. 12391 (2003) 118-127.
- Jellen, E.E.; Chappell, A.M., Ryzhov, V. Binding of Metalloporphyrins to Histidine-Containing Peptides and Model Systems: CID and IM Reactions. 50th ASMS Annual Conference, Orlando, FL.
- Jellen, E.E.; Ryzhov, V. Relative Binding Energies of Histidine, Lysine, and Arginine-Containing Peptides and Model Systems to Metalloporphyrins: A CID Experiment. 51st ASMS Annual Conference, Montreal, Quebec, Canada.
- Jellen, E.E. Weinecke, A.M. ; Ryzhov, Investigating the Reactivity of Metal Complexes with Benzene: IM Reactions. 52nd ASMS Annual Conference, Nashville, TN
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